Catalog Number - 85318-15P-2

The 85318‑15P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for maximum‑resolution separations in low‑flow LC‑MS workflows, delivering consistent, rugged performance across a wide pH range (2–12).

Featuring a bulky end‑capped C18 bonded phase on high‑purity silica, this column minimizes silanol interactions and improves peak shape for acidic, basic, and neutral analytes, especially those with extreme pKa values.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

150 mm Length – Maximum Resolution for LC‑MS Methods

• Highest resolving power in the 3 µm 2.1 mm ABX column range

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method optimization and validation workflows

✅ Selected when maximum resolution is required beyond 50 mm and 100 mm formats

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity through reduced solvent consumption

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise low‑flow pump delivery and low‑volume detection systems

⚠️ Narrow‑bore format requires accurate flow control for optimal results

3 µm Particle – Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Provides:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• LC‑MS systems without UHPLC pressure limits

• High‑performance HPLC workflows

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains integrity under aggressive analytical conditions

✅ Enables separations beyond standard C18 column limitations

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh methods

Balanced LC‑MS Performance

• Provides high efficiency without excessive backpressure

• Compatible with both HPLC and LC‑MS systems

Improved Peak Shape for Acids & Bases

• Advanced end‑capping reduces secondary interactions

• Enhances reproducibility and data quality

✅ Ideal for advanced method development and LC‑MS optimization

LC‑MS Performance Advantages

• Narrow‑bore 2.1 mm format:

  • Improves detection sensitivity

  • Reduces solvent usage

• Extended 150 mm length:

  • Maximizes resolution and peak capacity

  • Enables separation of highly complex mixtures

✅ Preferred configuration for high‑resolution LC‑MS workflows

System & Sample Considerations

• Designed for low‑flow LC‑MS and HPLC systems

• Requires:

  • Stable pump delivery at low flow rates

  • Low‑volume detector flow cells

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term performance and accuracy

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical method development and validation

• Stability‑indicating methods

• Peptide and polar compound separations

• Complex mixture resolution under extreme pH

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow systems (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution analytical workflows

• Ensure system capability for low‑flow precision and pressure tolerance